Inbred corn line ZS01595

ABSTRACT

Broadly this invention provides inbred corn line ZS01595. The methods for producing a corn plant by crossing the inbred line ZS01595 are encompassed by the invention. Additionally, the invention relates to the various parts of inbred ZS01595 including culturable cells. This invention relates to hybrid corn seeds and plants produced by crossing the inbred line ZS01595 with at least one other corn line.

FIELD OF THE INVENTION

This invention is in the field of corn breeding, specifically relatingto an inbred corn line designated ZS01595.

BACKGROUND OF THE INVENTION

The original maize plant was indigenous to the Western Hemisphere. Theplants were weedlike and only through the efforts of early breeders wasa cultivated crop species developed. The physical traits of maize aresuch that self pollination or cross pollination can occur. Each planthas a separate male and female flower, the tassel and ear, respectively.Natural pollination occurs when wind transfers pollen from tassel to thesilks on the corn ears. This type of pollination contributed to the widevariation of maize varieties present in the Western Hemisphere.

The development of a planned breeding program for maize only occurred inthe last century. Originally, maize was an open pollinated varietyhaving heterogeneous genotypes. The maize farmer selected uniform earsfrom the yield of these genotypes and reserved them for planting thenext season. The result was a field of maize plants that weresegregating for a variety of traits. This type of maize selection leadto at most incremental increases in seed yield.

Large increases in seed yield were the result of the development ofhybrid corn varieties in planned breeding programs. Hybrids weredeveloped by selecting corn lines and selfing these lines for severalgenerations to develop homozygous pure inbred lines and crossingselected inbred lines with unrelated inbred lines to produce hybridprogeny (F1). Inbred lines can be difficult to produce since theinbreeding process in corn decreases the vigor. However, when two inbredlines are crossed, the hybrid plant evidences greatly increased vigorcompared to open pollinated segregating maize plants. An importantfactor of the homozygosity and the homogeneity of the inbred lines isthat the hybrid from any cross will always be the same, and can bereproduced.

The ultimate objective of the commercial maize seed companies is toproduce high yielding, agronomically sound plants which perform well incertain regions or areas of the Corn Belt. To produce these types ofhybrids, the companies must develop inbreds which carry needed traitsinto the hybrid combination. Hybrids are not uniformly adapted for theCorn Belt, but are specifically adapted for regions of the Corn Belt.Northern regions of the Corn Belt require shorter season hybrids than dosouthern regions of the Corn Belt. Hybrids that grow well in Coloradoand Nebraska soils may not flourish in rich Illinois soil. Thus, avariety of major agronomic traits are important in hybrid combinationfor the various Corn Belt regions, and have an impact on hybridperformance.

Inbred line development and hybrid testing have been emphasized in thepast half century in commercial maize production as a means to increasehybrid performance. Inbred development is usually done by pedigreeselection. Pedigree selection can be selection in an F₂ populationproduced from a planned cross of two genotypes (often elite inbredlines), or selection of progeny of synthetic varieties, open pollinated,composite, or backcross populations. This type of selection is effectivefor highly inheritable traits, but other traits, for example, yieldrequires replicated test crosses at a variety of stages for accurateselection.

Maize breeders select for a variety of traits in inbreds that impacthybrid performance along with selecting for acceptable parental traits.Such traits include yield potential in hybrid combination; dry down;maturity; grain moisture at harvest; greensnap; resistance to rootlodging; resistance to stalk lodging; grain quality; disease and insectresistance; ear and plant height; performance in different soil typessuch as: low level of organic matter, clay, sand, black, high pH, lowpH; performance in: wet environments, drought environments, and notillage conditions. These traits appear to be governed by a complexgenetic system that makes selection and breeding of an inbred lineextremely difficult. Even if an inbred in hybrid combination hasexcellent yield (a desired characteristic), it may not be useful becauseit fails to have acceptable parental traits such as seed yield, seedsize, pollen production, good silks, plant height, etc.

To illustrate the difficulty of breeding and developing inbred lines,the following example is given. Two inbreds compared for similarity of29 traits differed significantly for 18 traits between the two lines. If18 simply inherited single gene traits were polymorphic with genefrequencies of 0.5 in the parental lines, and assuming independentsegregation (as would essentially be the case if each trait resided on adifferent chromosome arm), then the specific combination of these traitsas embodied in an inbred would only be expected to become fixed at arate of one in 262,144 possible homozygous genetic combinations.Selection of the specific inbred combination is also influenced by thespecific selection environment on many of these 18 traits which makesthe probability of obtaining this one inbred even more remote. Thus, thegeneral procedure of producing a non segregating F₁ generation and selfpollinating to produce a F₂ generation that segregates for traits doesnot easily lead to a useful inbred. Great care and breeder expertisemust be used in selection of breeding material to continue to increaseyield and agronomics of inbreds and resultant commercial hybrids.

SUMMARY OF THE INVENTION

The present invention relates to an inbred corn line ZS01595.Specifically, this invention relates to plants and seeds of this line.Additionally, this relates to a method of producing hybrid seed cornfrom this inbred. More particularly, this invention relates to theunique combination of traits that combine in corn line ZS01595.

Generally then, broadly the present invention includes an inbred cornseed designated ZS01595. This seed produces a corn plant.

The invention also includes the tissue culture of regenerable cells ofZS01595 wherein the tissue regenerates plants having the genotype ofZS01595. The tissue culture is selected from the group consisting ofleaves, pollen, embryos, roots, root tips, anthers, silk, flowers,kernels, ears, cobs, husks and stalks, and cells and protoplaststhereof. The corn plant regenerated from ZS01595 having ZS01595'sgenotype.

The invention extends to hybrid seed produced by planting, inpollinating proximity, seeds of corn inbred lines ZS01595 and anotherinbred line; cultivating corn plants resulting from said planting;preventing pollen production by the plants of one of the inbred lines;allowing natural cross pollinating to occur between said inbred lines;and harvesting seeds produced on plants of the inbred. The hybrid seedproduced by hybrid combination of plants of inbred corn seed designatedZS01595 and plants of another inbred line. Hybrid plants grown from thishybrid seed.

The invention further includes a method of hybrid F1 production. A firstgeneration (F1) hybrid corn plant produced by the process of planting,in pollinating proximity, seeds of corn inbred lines ZS01595 and anotherinbred line; cultivating corn plants resulting from said planting;preventing pollen production by the plants of one of the inbred lines;allowing natural cross pollinating to occur between said inbred lines;harvesting seeds produced on plants of the inbred; and growing aharvested seed.

A tissue culture of the regenerable cells of hybrid plants produced withuse of ZS01595 genetic material. A tissue culture of the regenerablecells of the corn plant produced by the method described above.

DEFINITIONS

In the description and examples which follow, a number of terms areused. In order to provide a clear and consistent understanding of thespecifications and claims, including the scope to be given such terms,the following definitions are provided.

BL MOIST

The moisture percentage of the grain at black layer, i.e., when 50% ofthe plants per plot have reached physiological maturity.

COLD GERM

Cold Germ is a measurement of seed germination under cold soilconditions. Data is reported as percent of seed germinating.

ECB

European corn borer a maize eating insect. ECBI is the first broodgeneration of European corn borers. ECBII is the second generation ofEuropean corn borers.

EMERGE

The number of emerged plants per plot (planted at the same seedlingrate) collected when plants have two fully developed leaves.

GI

This is a selection index which provides a single quantitative measureof the worth of a hybrid based on four traits. Yield is the primarytrait contributing to index values. The GI value is calculated bycombining stalk lodging, root lodging, yield and dropped ears accordingto the attached mathematical formula:

    GI=100+0.5(YLD)-0.9(%STALK LODGE)-0.9(%ROOT LODGE)-2.7(%DROPPED EAR)

GLS

Gray Leaf Spot (Cercospora Zeae) disease rating. This is rated on a 1-9scale with a "1" being very susceptible, and a "9" being veryresistant.*

GW

Goss' Wilt (Corynebacterium nebraskense). This is rated on a 1-9 scalewith a "1" being very susceptible, and a "9" being very resistant.*

HEATP10

The number of Growing Degree Units (GDU's) or heat units required for aninbred line or hybrid to have approximately 10 percent of the plantsshedding pollen. This trait is measured from the time of planting.Growing Degree Units are calculated by the Barger Method where the GDU'sfor a 24 hour period are: ##EQU1##

The highest maximum temperature used is 86° F. and the lowest minimumtemperature used is 50° F. For each inbred or hybrid it takes a certainnumber of GDU's to reach various stages of plant development.

HEATBL

The number of GDU's after planting when approximately 50 percent of theinbred or hybrid plants in a plot have grain which has reachedphysiological maturity (black layer).

HEATPEEK

The number of GDU's after planting of an inbred when approximately 50percent of the plants show visible tassel extension.

HEATP50 or HTP50

The number of GDU's required for an inbred or hybrid to haveapproximately 50 percent of the plants shedding pollen. Growing DegreeUnits are calculated by the Barger Method as shown in the HEATP10definition.

HEATP90

The number of GDU's accumulated from planting when the last 100 percentof plants in an inbred or hybrid are still shedding enough viable pollenfor pollination to occur. Growing Degree Units are calculated by theBarger Method as shown in the HEATP10 definition.

HEATS10

The number of GDU's required for an inbred or hybrid to haveapproximately 10 percent of the plants with silk emergence of at least0.5 inches. Growing Degree Units are calculated by the Barger Method asshown in the HEATP10 definition.

HEATS50 or HTS50

The number of GDU's required for an inbred or hybrid to haveapproximately 50 percent of the plants with silk emergence of at least0.5 inches. Growing Degree Units are calculated by the Barger Method asshown in the HEATP10 definition.

HEATS90

The number of GDU's required for an inbred or hybrid to haveapproximately 90 percent of the plants with silk emergence of at least0.5 inches. Growing Degree Units are calculated by the Barger Method asshown in the HEATP10 definition.

MDMV_(A)

Maize Dwarf Mosaic Virus strain A. The corn is rated on a 1-9 scale witha "1" being very susceptible, and a "9" being very resistant.*

MDMV_(B)

Maize Dwarf Mosaic Virus strain B. This is rated on a 1-9 scale with a"1" being very susceptible and a "9" being very resistant.*

MOISTURE

The average percentage grain moisture of an inbred or hybrid at harvesttime.

NLB

Northern Leaf Blight (Exserohilum turcicum) disease rating. This israted on a 1-9 scale with a "1" being very susceptible, and a "9" beingvery resistant.*

PCT TILLER

The total number of tillers per plot divided by the total number ofplants per plot.

PLANT

This term includes plant cells, plant protoplasts, plant cell tissuecultures from which corn plants can be regenerated, plant calli, plantclumps, and plant cells that are intact in plants or parts of plants,such as embryos, pollen, flowers, kernels, ears, cobs, leaves, husks,stalks, roots, root tips, anthers, silk and the like.

PLANT HEIGHT

The distance in centimeters from ground level to the base of the tasselpeduncle.

RM

Predicted relative maturity based on the moisture percentage of thegrain at harvest. This rating is based on known set of checks andutilizes standard linear regression analyses and is referred to as theMinnesota Relative Maturity Rating System.

SHED

The volume of pollen shed by the male flower rated on a 1-9 scale wherea "1" is a very light pollen shedder, a "4.5" is a moderate shedder, anda "9" is a very heavy shedder. The Table(s) 3 have reduced the 1-9 shedscale to a 1-3 shed scale. Any shed on Table 3 can be multiplied by 3 toreach the 1-9 shed scale.

SLB

Southern Leaf Blight (Bipolaris maydis) disease rating. This is rated ona 1-9 scale with a "1" being very susceptible, and a "9" being veryresistant.*

TWT

The measure of the weight of grain in pounds for a one bushel volumeadjusted for percent grain moisture.

VIGOR

Visual rating of 1 to 9 made 2∝3 weeks post-emergence where a "1"indicates very poor early plant development, and a "9" indicatessuperior plant development.

WARM GERM

A measurement of seed germination under ideal (warm, moist) conditions.Data is reported as percent of seeds germinating.

YIELD (YLD)

Actual yield of grain at harvest adjusted to 15.5% moisture.Measurements are reported in bushels per acre.

% DROPPED EARS (DE)

The number of plants per plot which dropped their primary ear divided bythe total number of plants per plot.

% LRG FLAT

Percentage by weight of shelled corn that passes through a 26/64 inchround screen and a 14/64 inch slot screen, but does not pass through ascreen with 20.5/64 inch round openings.

% LRG ROUND

Percentage by weight of shelled corn that passes through a 26/64 inchround screen, but does not pass through a 14/64 inch slot screen or ascreen with 20.5/64 inch round openings.

% MED FLAT

Percentage by weight of shelled corn that passes through a 20.5/64 inchround screen and a 13/64 inch slotted screen, but does not pass througha screen with 17/64 inch round openings.

% MED ROUND

Percentage by weight of shelled corn that passes through a 20.5/64 inchround screen, but does not pass through a 13/64 inch slot screen or ascreen with 17/64 inch round openings.

% SML FLAT

Percentage by weight of shelled corn that passes through a 17/64 inchround screen and a 12/64 inch slotted screen, but does not pass througha screen with 15/64 inch round openings.

% SML ROUND

Percentage by weight of shelled corn that passes through a 17/64 inchround screen, but does not pass through a 12/64 inch slotted screen or ascreen with 15/64 inch round openings.

% ROOT LODGE (RL)

Percentage of plants per plot leaning more that 30 degrees from verticaldivided by total plants per plot.

% STALK LODGE (SL)

Percentage of plants per plot with the stalk broken below the primaryear node divided by the total plants per plot.

DETAILED DESCRIPTION OF THE INVENTION

A B73 type line selected for oil content, along with a number of otherB73 lines, can be used as a female, in hybrid combination with ZS01595(which has a step level more of oil content in its kernels) generallyforms a good hybrid. ZS01595 which can be used as a male, due to itsgood pollen shed and plant height is the necessary male. Using ZS01595,in hybrid combination, results in hybrids with improved elevated levelsof protein and oil in the grain. The specific combining of ZS01595 witha B73 line selected for its increased oil content (about half apercentage point more than most B73 lines) is known as 24X. This hybridevidences itself as a robust hybrid of average yield ability withelevated percent oil and protein grain, further exhibiting very goodvigor and earlier maturity than competitive oil hybrids, such that the24X hybrid is suitable for production in more northerly growing zonesthan current hybrids which are classified as having elevated levels ofoil in the kernels.

The inbred has shown uniformity and stability within the limits ofenvironmental influence for all the traits as described in the VarietyDescription Information (Table 1) that follows. Most of the data in theVariety Description information was collected at Slater, Iowa or othercorn breeding research stations.

The inbred has been self-pollinated for a sufficient number ofgenerations to give inbred uniformity. During plant selection in eachgeneration, the uniformity of plant type was selected to ensurehomozygosity and phenotypic stability. The line has been increasedseparate in isolated farmland environments with data on uniformity andagronomic traits being observed to assure uniformity and stability. Novariant traits have been observed or are expected in ZS01595 or thehybrid 24X.

The best method of producing the invention, ZS01595 which issubstantially homozygous, is by planting the seed of ZS01595 which issubstantially homozygous and self-pollinating or sib pollinating theresultant plant in an isolated environment, and harvesting the resultantseed or the resultant pollen. The hybrid, 24X, is best produced byplanting the hybrid seed of 24X this hybrid seed is commerciallyavailable from Garst for production of the grain there from.

TABLE 1 VARIETY DESCRIPTION INFORMATION

#1 Type: Dent

#2 Region Best Adapted: GRM110 for grain in central and southernportions and certain western regions and for silage in northern andwestern portions as well as in central Michigan. Central and northernIowa and NE to N. Illinois, southern and western Minnesota, southwesternand northeastern Colorado.

    __________________________________________________________________________    INBRED ZS01595                                                                #3    MATURITY                                                                DAYS  HEATUNITS                                                               66    1609         FROM PLANTING TO 50% OF PLANTS IN SILK                     66    1609         FROM PLANTING TO 50% OF PLANTS IN POLLEN                   8                  FROM 10% TO 90% POLLEN SHED                                __________________________________________________________________________    #4    PLANT                                                                   DATA                                                                          2                  ANTHOCYANIN OF BRACE ROOTS: 1 = ABSENT 2 = FAINT 3 =                          MODERATE 4 = DARK                                          __________________________________________________________________________    #5    LEAF                                                                    COLOR/DATA                                                                    2/MEDIUM GREEN     LEAF COLOR **MUNSELL CODE-5 GY 4/4                         3                  LEAF SHEATH PUBESCENCE (1 = NONE TO 9 = PEACH FUZZ)        8                  MARGINAL WAVES (1 = NONE TO 9 = MANY)                      2                  LONGITUDINAL CREASES (1 = NONE TO 9 = MANY)                __________________________________________________________________________    #6    TASSEL                                                                  COLOR/DATA                                                                    5                  POLLEN SHED (0 = STERILE TO 9= HEAVY SHEDDER)              22/TAN             ANTHER COLOR **MUNSELL CODE-2.5 Y 7/8                      1/LIGHT GREEN      GLUME COLOR **MUNSELL CODE-2.5 GY 8/2 W/S 2.5 R 4/8        1                  BAR GLUME: 1 = ABSENT 2 = PRESENT                          __________________________________________________________________________    #7A   EAR (UNHUSKED DATA)                                                     COLOR/DATA                                                                    1/LIGHT GREEN      SILK COLOR (3 DAYS AFTER EMERGE) **MUNSELL CODE-2.5 GY                        8/7                                                        8/YELLOW-ORNGE     FRESH HUSK (25 DAYS AFTER 50% SILK) **MUNSELL CODE-2.5                        GY 7/10                                                    21/BUFF            DRY HUSK COLOR (65 DAYS AFTER 50% SILK **MUNSELL                              CODE-2.5 Y 8/4                                             2                  POSITION OF EAR AT DRY HUSK: 1 = UPRIGHT 2                                    = HORIZONTAL 3 = PENDENT                                   7                  HUSK TIGHTNESS (1 = VERY LOOSE TO 9 = VERY TIGHT)          3                  HUSK EXTENSION AT HARVEST: 1 = EXPOSED EAR 2 = 8 CM 3                         = 8-10 CM 4 = <10 CM                                       __________________________________________________________________________    #7B   EAR (HUSKED DATA)                                                       DATA                                                                          1                  KERNEL ROWS: 1 = INDISTINCT 2 = DISTINCT                   1                  ROW ALIGNMENT: 1 = STRAIT 2 = SLIGHT CURVE 3 = SPIRAL      2                  EAR TAPPER: 1 = STRAIT 2 = AVERAGE 3                       __________________________________________________________________________                       = EXTREME                                                  #8    KERNEL (DRY)                                                            COLOR/DATA                                                                    1                  ALEURONE COLOR PATTERN: 1 = HOMO 2 = SEG                   7/YELLOW           ALEURONE COLOR **MUNSELL CODE-2.5 Y 8/10                   8/YELLOW-ORNGE     HARD ENDOSPERM COLOR **MUNSELL CODE-7.5 YR 6/9             9                  ENDOSPERM TYPE                                             7/YELLOW           CROWN COLOR **MUNSELL CODE-2.5 Y 8/10                      __________________________________________________________________________    #9    COB                                                                     COLOR                                                                         19/WHITE           COB COLOR **MUNSELL CODE-5 Y 8/2                           __________________________________________________________________________    PROJECT MO PVP TRAITS                                                         INBRED ZS01595                                                                #10A                    N   MEAN  STD.  T-STAT PROB  95%                      __________________________________________________________________________                                                         CI                       EAR HEIGHT (CM)         15  65.07 10.02 25.16  0.0000                                                                              (60.00, 70.14)           LENGTH OF PRIMARY EAR LEAF (CM)                                                                       15  64.47 2.00  125.1  0.0000                                                                              (63.46, 65.48)           WIDTH OF PRIMARY EAR LEAF (CM)                                                                        15  7.37  0.97  29.35  0.0000                                                                              ( 6.87, 7.86)            TOP EAR INTERNODE (CM)  15  13.67 0.72  73.13  0.0000                                                                              (13.30, 14.03)           DEGREE OF LEAF ANGLE    13  25.13 3.40  28.64  0.0000                                                                              (23.41, 26.85)           # OF EARS PER PLANT     15  1.00  0.00               ( 1.00, 1.00)            # OF LEAVES ABOVE TOP EAR                                                                             15  6.80  0.77  34.00  0.0000                                                                              ( 6.41, 7.19)            # OF PRIMARY LATERAL TASSEL BRANCHES                                                                  15  10.27 1.03  38.50  0.0000                                                                              ( 9.74, 10.79)           PLANT HEIGHT (CM)       15  162.8 5.70  110.7  0.0000                                                                              (159.9, 165.7)           TASSEL LENGTH (CM)      15  41.33 2.23  71.93  0.0000                                                                              (40.21, 42.46)           TASSEL BRANCH ANGLE     15  9.33  4.81  7.52   0.0000                                                                              ( 6.90, 11.77)           # OF TILLER PER PLANTS  15  0.00  0.00               ( 0.00, 0.00)            WEIGHT PER 100 KERNELS (GM)                                                                           2   17.80 0.14  178.0  0.0036                                                                              (17.60, 18.00)           EAR LENGTH (CM)         2   14.00 0.00               (14.00, 14.00)           EAR WEIGHT (GM)         2   67.00 1.41  67.00  0.0095                                                                              (65.04, 68.96)           # OF KERNEL ROWS        2   17.00 1.41  17.00  0.0374                                                                              (15.04, 18.96)           COB DIAMETER AT MID-POINT (MM)                                                                        15  24.00 0.00               (24.00, 24.00)           EAR DIAMETER AT MID-POINT (MM)                                                                        2   38.00 1.41  38.00  0.0167                                                                              (36.04, 39.96)           KERNEL LENGTH (MM)      2   9.50  0.71  19.00  0.0335                                                                              ( 8.52, 10.48)           KERNEL THICKNESS (MM)   2   5.00  0.00               ( 5.00, 5.00)            KERNEL WIDTH (MM)       2   7.00  0.00               ( 7.00, 7.00)            % ROUND KERNELS(SHAPE GRADE)                                                                          2   47.00 4.24  15.67  0.0406                                                                              (41.12, 52.88)           SHANK LENGTH            2   11.33 3.42  12.85  0.0000                                                                              ( 9.60,                  __________________________________________________________________________                                                         13.06)                   PROJECT EB PVP TRAITS                                                         INBRED ZS01595                                                                #10B                    N   MEAN  STD.  T-STAT PROB  95%                      __________________________________________________________________________                                                         CI                       EAR HEIGHT (CM)         15  95.37 10.12 36.49  0.0000                                                                              (90.24, 100.5)           LENGTH OF PRIMARY EAR LEAF (CM)                                                                       15  72.13 2.39  117.1  0.0000                                                                              (70.93, 73.34)           WIDTH OF PRIMARY EAR LEAF (CM)                                                                        15  10.23 0.37  106.7  0.0000                                                                              (10.05, 10.42)           TOP EAR INTERNODE (CM)  15  14.53 0.48  117.1  0.0000                                                                              (14.29, 14.78)           DEGREE CF LEAF ANGLE    15  28.27 2.96  36.94  0.0000                                                                              (26.77, 29.77)           # OF EARS PER PLANT     15  1.20  0.41  11.22  0.0000                                                                              ( 0.99, 1.41)            # OF LEAVES ABOVE TOP EAR                                                                             15  7.47  0.52  56.00  0.0000                                                                              ( 7.21, 7.73)            # OF PRIMARY LATERAL TASSEL BRANCHES                                                                  15  15.27 2.63  23.94  0.0000                                                                              (14.94, 17.60)           PLANT HEIGHT (CM)       15  198.5 5.81  132.2  0.0000                                                                              (195.5, 201.4)           TASSEL LENGTH (CM)      15  37.70 2.31  63.34  0.0000                                                                              (36.53, 38.87)           TASSEL BRANCH ANGLE     15  17.67 5.29  12.94  0.0000                                                                              (14.99, 20.34)           # OF TILLER PER PLANTS  15  0.00  0.00               ( 0.00, 0.00)            WEIGHT PER 100 KERNELS (GM)                                                                           15  17.57 1.27  53.68  0.0000                                                                              (16.93, 18.22)           EAR LENGTH (CM)         15  14.64 0.65  86.93  0.0000                                                                              (14.31, 14.97)           EAR WEIGHT (GM)         15  99.42 8.44  45.62  0.0000                                                                              (95.15, 103.7)           # OF KERNEL ROWS        15  18.53 1.60  44.93  0.0000                                                                              (17.72, 19.34)           COB DIAMETER AT MID-POINT (MM)                                                                        15  24.29 1.22  77.17  0.0000                                                                              (23.68, 24.91)           EAR DIAMETER AT MID-POINT (MM)                                                                        15  38.41 1.74  85.67  0.0000                                                                              (37.53, 39.29)           KERNEL LENGTH (MM)      15  9.83  0.46  82.92  0.0000                                                                              ( 9.59, 10.06)           KERNEL THICKNESS (MM)   15  4.51  0.43  40.28  0.0000                                                                              ( 4.29, 4.73)            KERNEL WIDTH (MM)       15  7.36  0.49  58.22  0.0000                                                                              ( 7.11, 7.61)            % ROUND KERNELS(SHAPE GRADE)                                                                          15  28.53 5.41  20.42  0.0000                                                                              (25.79, 31.27)           SHANK LENGTH            15  14.17 3.97  13.81  0.0000                                                                              (12.16,                  __________________________________________________________________________                                                         16.18)               

The data provided above in #10A and #10B was from two (2) separatelocations and thus was provided as some environmental differences show.

#11 DISEASE RESISTANCE Northern leaf blight=5.5 Gray leaf spot=3.5MDMVB=1

#12 Inbreds comparable to ZS01595 are PVP8500037 (approved for Holden'sFoundation Seeds) and PVP8200062 (approved for Holden's FoundationSeeds) and hybrids comparable to 24X are 85391T (8532, 8692, 8481 IT).

The Munsell code is a reference book of color which is known and used inthe industry and by persons with ordinary skill in the art of plantbreeding.

The purity and homozygosity of inbred ZS01595 is constantly beingtracked using isozyme genotypes as shown in Table 2.

Isozyme Genotypes for ZS01595

Isozyme data were generated for inbred corn line ZS01595 according toprocedures known and published in the art. The data in Table 2 gives theelectrophoresis data on ZS01595.

                                      TABLE 2                                     __________________________________________________________________________    ELECTROPHORESIS RESULTS FOR ZS01595                                           __________________________________________________________________________    INBRED                                                                             ACP1                                                                              ACP4                                                                              ADH                                                                              MDH1                                                                              MDH2                                                                              PGD1                                                                              PGD2                                                                              PH1                                                                              PGM                                                                              IDH                                     ZS01595                                                                            33  66  22 22  11  11  11  22 22 11                                      __________________________________________________________________________

Inbred and Hybrid Performance of ZS01595

The traits and characteristics of inbred corn line ZS01595 are listed tocompare with other inbreds and/or in hybrid combination. ZS01595 datashows the characteristics and traits of importance, giving a snapshot ofZS01595.

Table 3A compares inbred ZS01595 with inbred PVP8500037. ZS01595 hassignificantly greater seedling vigor than UE95, and has significantlygreater plant and ear height. ZS01595 flowers later than PVP8500037,showing significantly greater GDUs at all HEATP and HEATS ratings.However, ZS01595 shows significantly lower grain moisture at harvest.Warm and cold germination ratings of ZS01595 are significantly lowercompared to PVP8500037. ZS01595 tends to produce larger kernels asevidenced by the significantly greater percent large round and flat, andpercent medium round kernel sizes, with a lower percent of the smallgrade kernels.

                                      TABLE 3A                                    __________________________________________________________________________    PAIRED INBRED COMPARISON DATA                                                 __________________________________________________________________________                             PCT    PLANT EAR          EAR   PCT                  YEAR  INBRED VIGOR EMERGE                                                                              TILLER HEIGHT                                                                              HEIGHT                                                                              SHED   QUALITY                                                                             BARREN               __________________________________________________________________________    OVERALL                                                                             Z501595                                                                              6.8   78.1         178.0 86.4  7.3                                     PVP8500037                                                                           5.2   84.6         128.7 57.6  7.5                                     # EXPTS                                                                              19    18           19    19    19                                      DIFF   1.6   6.5          49.3  28.8  0.2                                     PROB   0.000*                                                                              0.111        0.000*                                                                              0.000*                                                                              0.542                             __________________________________________________________________________    YEAR  INBRED HEATP10                                                                             HEATP50                                                                             HEATP90                                                                              HEATS10                                                                             HEATS50                                                                             HEATS90                           __________________________________________________________________________    OVERALL                                                                             ZS01595                                                                              1437  1477  1574   1465  1529  1575                                    PVP8500037                                                                           1379  1421  1511   1365  1421  1462                                    # EXPTS                                                                              19    19    19     19    19    19                                      DIFF   58    56    63     100   108   113                                     PROB   0.000*                                                                              0.000*                                                                              0.000* 0.000*                                                                              0.000*                                                                              0.000*                            __________________________________________________________________________                             BL     % ROOT                                                                              % STALK                                                                             % DROPPED                         YEAR  INBRED HEATPEEK                                                                            HEATBL                                                                              MOIST  LODGE LODGE EARS   MOISTURE                                                                            YIELD                __________________________________________________________________________    OVERALL                                                                             ZS01595                                                                              1346  2499  31.7                      11.2  39.8                       PVP8500037                                                                           1347  2475  34.0                      13.8  69.5                       # EXPTS                                                                              19    3     3                         19    19                         DIFF   1     25    2.3                       2.6   29.7                       PROB   0.941 0.190 0.401                     0.000*                     __________________________________________________________________________                 WARM  COLD  % LRG  % LRG % MED % MED  % SML % SML                YEAR  INBRED GERM  GERM  ROUND  FLAT  ROUND FLAT   ROUND FLAT                 __________________________________________________________________________    OVERALL                                                                             ZS01595                                                                              93.8  85.3  3.7    1.5   27.9  23.5   14.9  16.2                       PVP8500037                                                                           95.7  89.8  0.1    0.1   14.0  26.9   21.6  24.5                       # EXPTS                                                                              18    18    17     17    17    17     17    17                         DIFF   1.9   4.5   3.7    1.4   13.8  3.5    6.6   8.3                        PROB   0.048**                                                                             0.058***                                                                            0.003* 0.002*                                                                              0.000*                                  __________________________________________________________________________

Table 3B compares inbred ZS01595 with inbred PVP8200062. ZS01595 hassignificantly greater seedling vigor than PVP8200062, and hassignificantly greater plant and ear height. ZS01595 flowers earlier thanPVP8200062, showing significantly fewer GDUs at all HEATP and HEATSratings, as well as HEATPEEK. Warm and cold germination ratings ofZS01595 are significantly greater than PVP8200062. ZS01595 producesgrain with smaller kernel sizes as evidenced by the significantly lowerpercent large round and large flat kernel sizes. There are greaterpercent medium and small kernel sizes for ZS01595 compared toPVP8200062.

                                      TABLE 3B                                    __________________________________________________________________________    PAIRED INBRED COMPARISON DATA                                                 __________________________________________________________________________                             PCT    PLANT EAR          EAR   PCT                  YEAR  INBRED VIGOR EMERGE                                                                              TILLER HEIGHT                                                                              HEIGHT                                                                              SHED   QUALITY                                                                             BARREN               __________________________________________________________________________    OVERALL                                                                             Z501595                                                                              6.0   76.5         179.6 88.3  6.6                                     PVP8500037                                                                           5.0   81.2         160.7 81.1  6.2                                     # EXPTS                                                                              17    17           17    17    14                                      DIFF   1.0   4.7          18.8  7.2   0.4                                     PROB   0.000*                                                                              0.322        0.000*                                                                              0.025**                                                                             0.355                             __________________________________________________________________________    YEAR  INBRED HEATP10                                                                             HEATP50                                                                             HEATP90                                                                              HEATS10                                                                             HEATS50                                                                             HEATS90                           __________________________________________________________________________    OVERALL                                                                             ZS01595                                                                              1462  1506  1623   1528  1580  1639                                    PVP8500037                                                                           1580  1634  1751   1617  1671  1708                                    # EXPTS                                                                              15    15    15     15    15    15                                      DIFF   117   128   128    89    91    69                                      PROB   0.000*                                                                              0.000*                                                                              0.000* 0.000*                                                                              0.000*                                                                              0.001*                            __________________________________________________________________________                             BL     % ROOT                                                                              % STALK                                                                             % DROPPED                         YEAR  INBRED HEATPEEK                                                                            HEATBL                                                                              MOIST  LODGE LODGE EARS   MOISTURE                                                                            YIELD                __________________________________________________________________________    OVERALL                                                                             ZS01595                                                                              1363  2517  32.7                      11.6  38.8                       PVP8500037                                                                           1481  2625  29.5                      12.7  54.7                       # EXPTS                                                                              15    3     2                         16    17                         DIFF   117   108   3.2                       1.0   15.9                       PROB   0.000*                                                                              0.108 0.500                     0.138                      __________________________________________________________________________                 WARM  COLD  % LRG  % LRG % MED % MED  % SML % SML                YEAR  INBRED GERM  GERM  ROUND  FLAT  ROUND FLAT   ROUND FLAT                 __________________________________________________________________________    OVERALL                                                                             ZS01595                                                                              91.9  80.7  3.5    1.8   27.9  25.8   17.9  14.6                       PVP8500037                                                                           83.7  57.2  37.6   27.0  21.8  10.3   1.4   0.8                        # EXPTS                                                                              15    15    14     14    14    14     14    13                         DIFF   8.2   23.5  34.1   25.2  6.1   15.5   16.5  13.9                       PROB   0.036**                                                                             0.000***                                                                            0.000* 0.000*                                                                              0.103                                   __________________________________________________________________________

Table 4 shows the GCA (general combining ability) estimates ofZS01595compared with the GCA estimates of the other inbreds. Theestimates show the general combining ability is weighted by the numberof experiment/location combinations in which the specific hybridcombination occurs. The interpretation of the data for all traits isthat a positive comparison is a practical advantage. A negativecomparison is a practical disadvantage. The general combining ability ofan inbred is clearly evidenced by the results of the general combiningability estimates. This data compares the inbred parent in a number ofhybrid combinations to a group of "checks". The check data is from othercompanies' hybrids, particularly leaders in the industry and ICI Seeds'commercial products and pre-commercial hybrids which were grown in thesame sets and locations.

Table 4A shows ZS01595 crossed in 439 hybrid combinations. ZS01595 onlyshows a positive advantage in test weight, and negative for otherperformance characteristics. The value of ZS01595 in hybrid combinationis that it is an average performer across a number of environments butthis line carries into its hybrids elevated oil and protein contain ofthe kernels. This oil and protein data is shown below as well as thehybrid data for 24X.

                                      TABLE 4A                                    __________________________________________________________________________    GCA & SCA ESTIMATES                                                           ADVANTAGES OVER COMMERCIALS & N'S                                             N93    N94 N95                                                                              N   FI  Y M   GI I   YLD                                                                              MST                                                                              % SL                                                                              % RL                                                                              % DE                                                                              TWT                                                                              POP RM                __________________________________________________________________________    XR= 75 3143                                                                              1229                                                                             4474                                                                              -2.4                                                                              -0.3  -0.9                                                                             -1.0                                                                              -1.2                                                                             -0.7                                                                             -0.2                                                                              -0.3                                                                              0.1 0.5                                                                              1   112               XH=           86  -5.7                                                                              -1.5  -4.7                                                                             -4.2                                                                              -7.1                                                                             -1.4                                                                             -1.4                                                                              -0.0                                                                              0.0 0.2                                                                              -103                                                                              110               XT=           19  -3.6                                                                              -0.3  -2.2                                                                             -1.7                                                                              -2.2                                                                             -0.6                                                                             -1.2                                                                              -0.2                                                                              0.1 0.3                                                                              2   111               XR= 314                                                                              8   110                                                                              439 -8.1                                                                              -0.6  -6.9                                                                             -0.7                                                                              -10.9                                                                            -0.5                                                                             -1.4                                                                              -0.2                                                                              0.0 0.1                                                                              -27 118               XH=           27  -8.8                                                                              -0.8  -7.4                                                                             -0.3                                                                              -13.7                                                                            -0.6                                                                             -0.7                                                                              -0.0                                                                              0.0 -0.1                                                                             23  120               XT=           2   -6.5                                                                              -0.2  -5.8                                                                             -0.7                                                                              -5.4                                                                             -0.3                                                                             -2.9                                                                              -0.6                                                                              0.0 0.8                                                                              -223                                                                              113               __________________________________________________________________________     XR= GCA ESTIMATE: WEIGHTED BY EXPT                                            XH= GCA ESTIMATE: WEIGHTED BY PARENT2                                         XT= SAME AS XH, BUT USING ONLY THOSE PARENT2 WITH TWO YEARS OF DATA      

Table 4B compares ZS01595 in random hybrid combinations with PVP8200062and PVP8500037 in random combinations. ZS01595 shows an unfavorableadvantage compared to either other inbred. The value of ZS01595 is itscontribution to elevated oil and protein levels in hybrid combination,and not its competitiveness as a normal corn inbred selected forcombining ability.

                  TABLE 4B                                                        ______________________________________                                                       N       FI   YM   GI   I    YLD  MST                           ______________________________________                                        ZS01595 XR =   4474    -2.4 -0.3 -0.9 -1.0 -1.2 -0.7                          PVP8200062                                                                            XR =   13352   -2.3 -0.3 -0.4 -0.6 -0.2 -0.8                          PVP8500037                                                                            XR =   11656   -2.7 -0.3 -2.3-                                                                              -2.2 -4.1 -0.2                                         % SL    % RL % DE   TWT  POP  RM                               ______________________________________                                        ZS01595 XR =   -0.2    -0.3 0.1    0.5  1    112                              PVP8200062                                                                            XR =   -0.1    -0.2 0.0    0.3  -34  123                              PVP8500037                                                                            XR =   -0.2    -0.2 0.0    0.5  5    113                              ______________________________________                                    

Table 4C shows the values for percent oil and percent protein of inbredgrain grown in different years. ZS01595 shows consistently elevated oiland protein levels across years compared to PVP8500037.

                  TABLE 4C                                                        ______________________________________                                        OIL AND PROTEIN DATA AND INBRED GRAIN                                                  1st year     2nd year                                                INBRED     % Oil  % Protein   % Oil                                                                              % Protein                                  ______________________________________                                        ZS01595    5.38   13.99       5.35 17.03                                      PVP8500037 3.67   10.66       4.36 13.87                                      ______________________________________                                    

Table 5 shows that ZS01595 in hybrid combination as 24X in comparisonwith the plants in the environment around it at the same location.ZS01595 in hybrid combination yields well in low to medium yieldingenvironments. In high yielding environments, the 24X hybrid tends toshow less aggressive yields compared to the environment.

                  TABLE 5                                                         ______________________________________                                        YIELD RESPONSE                                                                HYBRID    YIELD                                                               ______________________________________                                        24X       141       157    174    190  206                                    Environment                                                                             139       157    175    193  211                                    ______________________________________                                    

Table 6A.1 shows the advantage the 24X hybrid has compared to two (2)commercially available ICI Seeds' hybrids. The 24X hybrid is generallycompetitive in yield/moisture and shows a positive moisture advantageand a negative percent stalk lodge compared to both 8539IT and 8481 IT.Compared to 8481 IT and 8539IT, 24X continues to carry testweight intothe hybrid.

                                      TABLE 6A.1                                  __________________________________________________________________________    HYBRID SUMMARY                                                                24 X HYBRID OF ZS01595 INBRED                                                 PERFORMANCE DATA                                                              HYBRID                                                                              N  FI GI Y/M YLD                                                                              MST                                                                              % SL                                                                             % RL                                                                             % DE                                                                              TWT                                        __________________________________________________________________________    RE 8539IT                                                                           69 -3.0                                                                             -4.0                                                                             -0.0                                                                              -2.3                                                                             0.3                                                                              -2.4                                                                             -0.5                                                                             0.0 0.6                                        RE 8481IT                                                                           24 -0.8                                                                             -3.9                                                                             0.5 0.4                                                                              1.4                                                                              -4.6                                                                             0.0                                                                              0.0 0.4                                        __________________________________________________________________________     *Significant different at the 0.1 level                                  

Table 6A.2 shows the percent oil and protein for 24X compared to 8539ITand 8481 IT. 24X has significantly higher percent oil than 8539IT and8481 IT, and significantly higher protein than 8539IT.

                  TABLE 6A.2                                                      ______________________________________                                        PAIRED COMPARISONS OF PERCENT OIL AND PROTEIN                                        N         % OIL   % PROTEIN                                            ______________________________________                                        24X      4           4.51*   10.57*                                           8539IT               4.11    9.23                                             24X      2           4.15*   10.75                                            8481IT               3.05    9.10                                             ______________________________________                                    

Table 6B.1 shows the advantages and disadvantages generated bycomparison of the agronomic data of the two hybrids with 24X hybrid. The24X hybrid is taller and has higher ear placement than either 8539IT or8481 IT. Most agronomic traits are slightly less competitive; i.e., showa negative advantage, for 24X hybrid. The 24X hybrid silks, sheds pollenand reaches black layer at higher GDUs than either commercial hybrid.

                  TABLE 6B.1                                                      ______________________________________                                        ZS01595/INBRED                                                                AGRONOMIC DATA                                                                                             PLANT  EAR    STAY-                              HYBRID  N     ESTAND   VIGOR HEIGHT HEIGHT GREEN                              ______________________________________                                        8539IT  17    -6.3     -0.3  12.5   5.0    -0.7                               8481IT  5     -6.3     -0.6  18.4   9.1    -0.1                               ______________________________________                                    

Table 6B.2 shows the insect rating for ECB1 and ECB2. Additionally, theplant integrity score is indicated.

                  TABLE 6B.2                                                      ______________________________________                                                                    EAR    PLANT                                              ECB1     ECB2       DAMAGE INTEGRITY                                  INBRED  RATING   RATING     RATING RATING                                     ______________________________________                                        ZS01595 2.50     1.32       1.25   5.5                                        ______________________________________                                    

Table 6C.1 shows that the percent oil and protein levels of 24X areconsistently elevated compared with normal corn hybrids across years.

                                      TABLE 6C.1                                  __________________________________________________________________________    OIL AND PROTEIN DATA OF 24X                                                   COMPARED TO NORMAL CORN HYBRIDS                                                        % Oil          % Protein                                                      LOCS                                                                              (@ 0% H2O) LOCS                                                                              (@ 0% H2O)                                        __________________________________________________________________________    One                                                                              Experimental                                                                        3   5.5        1   15.0                                              Year                                                                             24X                                                                           Avg of    4.6    (Range  12.1   (Range                                        Normal C's       4.4-4.6)       12.0-12.1)                                 One                                                                              Experimental                                                                        4   4.8        4   13.1                                              Year                                                                             24X                                                                           Avg of    3.6    (Range                                                                            4   10.3   (Range                                        Normal C's       3.1-4.1)       8.6-12.0)                                  Two                                                                              Experimental                                                                        7   5.1        5   13.5                                              Years                                                                            24X                                                                           Avg of    4.0            10.7                                                 Normal C's                                                                 __________________________________________________________________________

Table 6D.1 shows the advantages and disadvantages of 24X compared tothree competitor commercial oil hybrids for hybrid performance. The 24Xhybrid shows a yield disadvantage compared to PFKERNOIL4, but a yieldadvantage compared to PFKERNOIL6 and PFKERNOIL8. The 24X hybrid shows apositive advantage for moisture, percent root lodging and testweight,and a negative disadvantage for percent stalk lodging compared to allthree PFKERNOIL hybrids.

                  TABLE 6D.1                                                      ______________________________________                                        Vs.      Year   #      Yield                                                                              Moisture                                                                             % SL  % RL TwT                             ______________________________________                                        PFKERNOIL4                                                                             N      25     -3   3.6    -5.9  1.6  2.3                             PFKERNOIL6                                                                             N      25     9    2.6    -4.2  0.0  0.6                             PFKERNOIL8                                                                             N      25     4    4.2    -5.5  3.7  2.2                             ______________________________________                                    

Table 6D.2 shows the percent oil and protein levels of 24X compared toPFKERNOIL hybrids. The 24X hybrid's percent oil ranges from 0.7-1.8percent lower than various PFKERNOIL hybrids, and 24X percent protein is2.0-3.7 percent higher than the PFKERNOIL hybrids.

                  TABLE 6D.2                                                      ______________________________________                                                     % Oil      % Protein                                                                (@               (@                                                     LOCS  0% H2O)  LOCS    0% H2O)                                   ______________________________________                                        One     Experimental                                                                             2       5.4                                                Year    24X                                                                           PFKERNOIL4         7.2                                                        PFKERNOIL6         7.2                                                        PFKERNOIL8         7.1                                                One     Experimental                                                                             4       4.8    4     13.1                                  Year    24X                                                                           PFKERNOIL4         5.5          9.4                                           PFKERNOIL6         5.9          11.0                                          PFKERNOIL8         5.9          11.1                                  Two     Experimental                                                                             6       5.0                                                Years   24X                                                                           PFKERNOIL4         6.0                                                        PFKERNOIL6         6.3                                                        PFKERNOIL8         6.3                                                ______________________________________                                    

Table 6E shows the silage data for 24X hybrid compared to TMF106, acompetitor silage hybrid. 24X hybrid produces comparable yield tonnageand net energy for lactation per acre (NE/Lact/acre) per acre (TN/A) asTMF106. 24X has a greater crude protein (CP) value, lower acid detergentfiber (ADF) and neutral detergent fiber (NDF) values, and greater valuefor total digestible nutrients (TDN). Thus, 24X is comparable or betterthan competitive commercial silage hybrids.

                  TABLE 6E                                                        ______________________________________                                        LOCS        TN/A   CP     ADF  NDF  TDN  NE/Lact/acre                         ______________________________________                                        Experimental                                                                          7       19.5   8.5  21.2 44.6 73.0 10,860                             24 X                                                                          TMF106          19.9   8.0  23.3 46.9 71.4 10,802                             ______________________________________                                    

The inbred ZS01595 can be employed as the male plant in a hybridproduction field to produce the hybrid 24X. This inbred is a tall inbredwith moderately high ear placement. ZS01595 is medium green color duringthe growing season and has an ear with indistinct kernel rows and awhite cob.

ZS01595, as an inbred line in hybrid combination of 24X, shows goodspecific combining ability. The hybrid 24X has average yield potentialcompared to normal commercial hybrids, but has a yield advantage and adistinct moisture advantage compared to commercial competitor oilhybrids. As a result, 24X is adapted and performs well as a grain hybridwith elevated oil and protein levels in more northern corn growingregions than competitor oil hybrids. Due to its elevated oil and proteinlevels, 24X may have value as an improved grain for monogastric (i.e.poultry, swine) animal feed.

Hybrid 24X also exhibits good silage feed characteristics. 24X producestonnage, protein, TDN and net energy available for lactation/acrecomparable or higher than competitor hybrid (TMF106) recognized for itssilage quality. Thus, 24X has value as a silage hybrid for dairy cows.

Hybrid 24X is versatile and can be used as a grain hybrid as well as alo silage hybrid due to its moisture and yield advantage over competitoroil and silage hybrids.

The foregoing is set forth by way of example and is not intended tolimit the scope of the invention.

This invention also is directed to methods for producing a corn plant bycrossing a first parent corn plant with a second parent corn plantwherein the first or second parent corn plant is an inbred corn plantfrom the line ZS01595. Further, both first and second parent corn plantscan come from the inbred corn line ZS01595. A variety of breedingmethods can be selected depending on the mode of reproduction, thetrait, the condition of the germplasm. Thus, any such methods using theinbred corn line ZS01595 are part of this invention: selfing,backcrosses, hybrid production, crosses to populations, haploid andanther culturing and the like.

Various culturing techniques known to those skilled in the art, such ashaploid, transformation, and a host of other conventional andunconventional methods are within the scope of the invention. All plantsand plant cells produced using inbred corn line ZS01595 are within thescope of this invention. The invention encompasses the inbred corn lineused in crosses with other, different, corn inbreds to produce (F1) cornhybrid seeds and plants with the characteristics that make good hybrids.This invention includes cells which upon growth and differentiationproduce corn plants having the physiological and morphologicalcharacteristics of the inbred line ZS01595.

Duncan, from at least 1985-1988 produced literature on plantregeneration from callus. Both inbred and hybrid callus have resulted inregenerated plants at excellent efficiency rates. Somatic embryogenesishas been performed on various maize tissue such as glume which beforethe 1980's was considered unusable for this purpose. The prior artclearly teaches the regeneration of plants from various maize tissues.

European Patent Application, publication 160,390, describes tissueculture of corn which can be used by those skilled in the art. Corntissue culture procedures are also described in the literature as earlyas 1982.

A deposit of at least 2500 seeds of the inbred seed of this invention ismaintained by ICI Seeds, 2369 330th Street, Slater, Iowa 50244. Adeposit of at least 2500 seeds of the hybrid seed (which is commerciallyavaialbe and therefor deposit of this material is presumed unnecessary)of this invention is maintained by ICI Seeds, 2369 330th Street, Slater,Iowa 50244. Access to these deposits will be available during thependency of this application to the Commissioner of Patents andTrademarks and persons determined by the Commissioner to be entitledthereto upon request. All restrictions on availability to the public ofsuch inbred material will be removed upon allowance of any claims ofthis application by depositing at least 2500 seeds of this invention atthe American Type Culture Collection, Rockville, Md. The Applicant madea deposit on Jun. 11, 1997, of at least 2500 seeds of Inbred Corn LineZS01595 with the American Type Culture Collection (ATCC), located at12301 Parklawn Drive, Rockville, Md. 20852. The ATCC accession number is209114. Additionally the Applicant has satisfied all of the requirementsof C.F.R.§1.801-1.809, including providing an indication of theviability of the sample. The ATCC deposit will be maintained in thatdepository, which is a public depository, for a period of 30 years, or 5years after the last request, or for the effective life of the patent,whichever is longer, and will be replaced if it becomes nonviable duringthat period.

Inbreds designated MBS are available from Mike Brayton Seed in Iowa.Inbreds designated SGI are available from Seed Genetic Inc. in NewJersey. Information on some ZS designations may be available from thePVP office.

Accordingly, the present invention has been described with some degreeof particularity directed to the preferred embodiment of the presentinvention. It should be appreciated, though, that the present inventionis defined by the following claims construed in light of the prior artso that modifications or changes may be made to the preferred embodimentof the present invention without departing from the inventive conceptscontained herein.

We claim:
 1. Inbred corn seed designated ZS01595, some of said seeddeposited in the ATCC and carry accession number
 209114. 2. A corn plantproduced by the seed of claim
 1. 3. A tissue culture of regenerablecells of ZS01595 of claim 1 wherein the cells of the tissue cultureregenerate plants having the genotype of ZS01595.
 4. A tissue cultureaccording to claim 3, the tissue culture selected from the groupconsisting of leaves, pollen, embryos, roots, root tips, anthers, silk,flowers, kernels, ears, cobs, husks and stalks, and cells andprotoplasts thereof.
 5. A corn plant having the genotype of ZS01595regenerated from the cells of the tissue culture of claim
 3. 6. Hybridseed produced by:(a) planting, in pollinating proximity, seeds accordingto claim 1 of corn inbred lines ZS01595 and another inbred line, one ofsaid inbred lines not releasing pollen; (b) cultivating corn plantsresulting from said planting; (c) allowing natural cross pollinating tooccur between said inbred lines; and (d) harvesting seeds produced onthe non pollen releasing inbred.
 7. Hybrid seed produced by hybridcombination of plants of inbred corn seed designated ZS01595 in claim 1and plants of another inbred line.
 8. Hybrid plants grown from seed ofclaim
 7. 9. A first generation (F1) hybrid corn plant produced by usingZS01595 according to claim 1 the process of:(a) planting, in pollinatingproximity, seeds of corn inbred lines ZS01595 and another inbred line;(b) cultivating corn plants resulting from said planting; (c) preventingpollen production by the plants of one of the inbred lines; (d) allowingnatural cross pollinating to occur between said inbred lines; (e)harvesting seeds produced on plants of the inbred line of step (c); and(f) growing a harvested seed of step (e).
 10. A tissue culture of theregenerable cells of the corn plant of claim
 8. 11. A tissue culture ofthe regenerable cells of the corn plant of claim 9.